Cespe UnB

Editorial Assistants:
W. Abrahão
G. Oliveira
L. Salgueiro

Editorial Technical Support:
D. H. Diaz
M. A. Gomez
J. Barbosa

Editorial management and production:
SOLGRAF Editora
solgraf@gmail.com






95/105= 0.91


1,1

Recovery from stress: A cell cycle perspective.

doi: 10.6062/jcis.2012.03.01.0049 (Free PDF)

Authors

Radmaneshfar E. and Thiel M.

Abstract

We develop a Boolean model to explore the dynamical behaviour of budding yeast in response to osmotic and pheromone stress. Our model predicts that osmotic stress halts the cell cycle progression in either of four possible arrest points. The state of the cell at the onset of the stress dictates which arrest point is finally reached. According to our study and consistent with biological data, these cells can return to the cell cycle after removal of the stress. Moreover, the Boolean model illustrates how osmotic stress alters the state transitions of the cell. Furthermore, we investigate the influence of a particular pheromone based method for the synchronisation of the cell cycles in a population of cells. We show this technique is not a suitable method to study one of the arrest points under osmotic stress. Finally, we discuss how an osmotic stress can cause some of the so called frozen cells to divide. In this case the stress can move these cells to the cell cycle trajectory, such that they will replicate again.

Keywords

Boolean network, state transition, cell cycle, stress response, osmotic stress, alpha factor.

References

[1] LI F, LONG T, LU Y, OUYANG Q & TANG C. 2004. The yeast cellcycle network is robustly designed. PNAS, 101(14): 4781–4786.

[2] BREEDEN LL. 1997. Alpha-factor synchronisation of budding yeast. Methods in Enzymology, 283: 332–341.

[3] SURANA U, ROBITSCH H, PRICE C, SCHUSTER T, FITCH I, FUTCHER B & NASMYTH K. 1991. The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae . Cell, 65(1): 145–161.

[4]SIA R, HERALD H & LEW DJ. 1996. Cdc28 tyrosine phosphorylation and the morphogenesis checkpoint in budding yeast. Molecular Biology of the Cell, 7(11): 1657–1666.

[5]AMON A, TYERS M, FUTCHER B & NASMYTH K. 1993. Mechanisms that help the yeast cell cycle clock tick: G2 cyclins transcriptionally activate G2 cyclins and repress G1 cyclins. Cell, 74(6):993–1007.

[6] STEGMEIER F & AMON A. 2004. Closing mitosis: The functions of the Cdc14 phosphatase and its regulation. Annual Review of Genetics, 38: 203–232.

[7] VISINTIN R, PRINZ S & AMON A. 1997. CDC20 and CDH1:a family of substrate-specific activators of APC-dependent prote- olysis. Science, 278(5337): 460–463.

[8] BÄUMER M, BRAUS GH & IRNIGER S. 2000. Two different modes of cyclin clb2 proteolysis during mitosis in Saccharomyces cerevisiae . FEBS Letters, 468(2-3): 142–148.

[9] DE NADAL E, ALEPUZ PM & POSAS F. 2002. Dealing with osmostress through MAP kinase activation. EMBO reports, 3(8): 735–740.

[10] BREWSTER JL, DE VALOIR T, DWYER ND, WINTER E & GUSTIN MC. 1993. An osmosensing signal transduction pathway in yeast. Science, 259(5102): 1760–1763.

[11] BELLÍ G, GARÍ E, ALDEA M & HERRERO E. 2001. Osmotic stress causes a G1 cell cycle delay and downregulation of Cln3/Cdc28 activity in Saccharomyces cerevisiae . Molecular Microbiology, 39(4): 1022–1035.

[12] ESCOTE X, ZAPATER M, CLOTET J & POSAS F. 2004. Hog1 mediates cell-cycle arrest in G1 phase by the dual targeting of Sic1. Nature Cell Biology, 6(10): 997–1002.

[13] ZAPATER M, CLOTET J, ESCOTE X & POSAS F. 2005. Control of Cell Cycle Progression by the Stress-Activated Hog1 MAPK. Cell Cycle: 6–7. [14] YAAKOV G, DUCH A, GARCÍA-RUBIO M, CLOTET J, JIMENEZ J, AGUILERA A & POSAS F. 2009. The stress-activated protein kinase Hog1 mediates S phase delay in response to osmostress. Molecular Biology of the Cell, 20(15): 3572–3582.

[15] CLOTET J, ESCOTÉ X, ADROVER M, YAAKOV G, GARÍ E, ALDEA I M, DE NADAL E & POSAS F. 2006. Phosphorylation of Hsl1 by Hog1 leads to a G2 arrest essential for cell survival at high osmo larity. The EMBO Journal, 25(11): 2338–2346.

[16] MENDENHALL MD & HODGE AE. 1998. Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae . Microbiology and Molecular Biology Reviews, 62(4): 1191–1243.

[17] ROBERT F. 1986. Discrete Iterations: A Metric Study. Springer Series in Computational Mathematics.

[18] TYERS M, TOKIWA G & FUTCHER B. 1993. Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins. The EMBO Journal, 12(5): 1955–1968.

[19] REISER V, D’AQUINO KE, LY-SHA E & AMON A. 2006. The stress activated mitogen-activated protein kinase signaling cascade pro- motes exit from mitosis. Molecular Biology of the Cell, 17(7): 3136–3146.

Search