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1,1

Computational analysis of mitochondrial genomic sequences in Dermatophytes for the identification of conserved coil functional residues.

doi: 10.6062/jcis.2013.04.03.0077

Authors

B. Gupta and J. Kaur

Abstract

Dermatophyte is a group of closely related fungi that have the capacity to invade keratinized tissue of humans and other animals, causing an infection, known as dermatophytosis. The identification of evolutionary relationship between three genera of dermatophyte is epidemiologically important to understand their pathogenicity. Mitochondrial genome reported to be less affected by genetic recombination, shows a higher rate of evolution and offer many advantages for phylogenetic studies. Thus, here we present a novel scheme to identify the conserved coil functional residues of Epidermophyton floccosum, Microsporum canis, Trichophyton mentagrophytes and Trichophyton rubrum. For the identification of such residues, mitochondrion genomic analysis was done using various tools and software’s viz. BLAST, Clustal W2, MODELLER 9v6, ORF Finder, SPDB Viewer, GOR V and LIGSITE. Protein coding sequences of the mitochondria genome, retrieved from GenBank were aligned for the similar sequences. For secondary structure, homology modelling was performed and pockets were identified using Ligsite. These results were then compared with GOR V and MSA results. Comparative analysis of the protein sequences revealed the presence of functionally active sites. However absence of such sites in Epidermophyton floccosum, could be responsible for its lesser predominance and infectivity as compared to other two genera. The functional coil residues identified can further be used for studying the protein-protein interactions and act as the target molecules for drug designing.

Keywords

Dermatophyte, Genome, Homology, Protein structure, Protein sequences.

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