Editorial Office:
Management:
R. S. Oyarzabal
Technical Support:
D. H. Diaz
M. A. Gomez
W. Abrahão
G. Oliveira
Publisher by Knobook Pub
Editorial Office:
Management:
R. S. Oyarzabal
Technical Support:
D. H. Diaz
M. A. Gomez
W. Abrahão
G. Oliveira
Publisher by Knobook Pub
doi: 10.6062/jcis.2012.03.02.0055(Free PDF)
Anielski A., Pfannes E. K. B., and Beta, C.
Many cellular processes involve the translocation of proteins from the cytosolic region to the cortex of the cell and vice versa. The dynamics of such processes is typically investigated by fluorescence imaging of GFP-labeled versions of these proteins. Quantitative analysis of the resulting fluorescence images requires image segmentation procedures that identify the cell within the image and subsequently divide the cell into a cytosolic and a cortical region to monitor the temporal evolution of the fluorescence signals within these parts of the cell separately. Here, we present an image segmentation protocol that we have developed for this type of data analysis. It consists of noise reduction, normalization, and thresholding steps to generate masks that define the cytosolic and the cortical regions of a cell. Based on these masks, the desired fluorescence signals can be extracted from the confocal microscopy images.
fluorescence microscopy, image processing, Dictyostelium.
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